Genotypic Information

From a composite set of more than 3000 accessions, a reference set of 384 accessions was then defined for allele mining which captured 78% of alleles (615 alleles) produced in composite germplasm collection. This set consisting of 23 wild, 4 advanced cultivars, 25 breeding materials and 332 landraces. At present seed for 375 accessions are available. The average gene diversity among the reference set is 0.713 with an average PIC value 0.679, which is equal to global composite collection.  

The SSR markers used to genotype the composite genebank collection by which the reference collection is derived are available. The reference kit results from the formation of the Sorghum Reference Set from a composite set of  3384 sorghum accessions and is composed of 48 SSRs with a sequence list, and allele sizes of markers.  Access the detailed list at http://sorghum.cirad.fr/SSR_kit/.

Supporting Information

1. Claire Billot, Ronan Rivallan, Mbaye Ndoye Sall, DanielL Fonceka, Monique Deu, Jean-Christophe Glaszmann, Jean-Louis Noyer, Jean-Francois Rami, Ange-Marie RisterrucciI, Patick Wincker, Puna Ramu and Charles T. Hash. A reference microsatellite kit to assess for genetic diversity of Sorghum bicolor (Poaceae), in American Journal of Botany: e245–e250. 2012. http://oar.icrisat.org/5946/7/e245.full.pdf
2. Billot C., Ramu P., Bouchet S., Chantereau J., Deu M. et al., 2013. Massive sorghum collection genotyped with SSR markers to enhance use of global genetic resources, in PLoS One, 8: e59714. Doi:10.1371/journal.pone.005
3. Mutegi , E. , F. Sagnard , K. Semagn , M. Deu , M. Muraya , B. Kanyenji , S. De Villiers , Et Al. 2011 Genetic structure and relationships within and between cultivated and wild sorghum ( Sorghum bicolor (L.) Moench) in Kenya as revealed by microsatellite markers, in Theoretical and Applied Genetics 122 : 989 – 1004. See on the Springer website.